Pre-Conference Hands

29th AMBICON Pre-Conference Workshops 18th – 20th July 2022

About the workshop: Recent developments in molecular methods have revolutionized the detection and characterization of various genes in humans and microorganisms and aid in future research. Among these methods, Polymerase Chain Reaction (PCR) has generated great benefits and allowed scientific advancements. PCR is an excellent technique for the rapid detection of biomarkers or therapeutic targets by amplifying the specific sequence of DNA. This workshop aims to outline the principles and applications of conventional PCR techniques in medical sciences. We will also discuss the indications, uses, and advantages of these techniques, as well as their advances in various medical areas. In addition to hands-on training, the on-topic lecture by subject experts allows the students to acquire theoretical knowledge about the methods.

Topic Venue Company-sponsored
Basic Molecular Biology Workshop
Day 1 Isolation of DNA Department of Biochemistry, Ramaiah Medical College Eppendorf
Day 2 Primer designing and Preparation of Master Mix, PCR
Day 3 Gel electrophoresis to check quality and nanodrop to estimate the quantity of DNA

1 Dr Aarathy D 9487558737
2 Dr Abhinya N Sudheer 9895077204
3 Dr Anitha P Rao 6361506293
4 Dr Anupama BS 9497689916
5 Dr Aravind Raj R 7094830708
6 Dr Bhavya S 9985500800
7 Dr Cukku Sara Benny 8547705475
8 Dr Diksha Malik 9350538574
9 Dr Haseena Shaik 9848041781
10 Dr Diana ferdinend 9962069145
11 Dr Manaswini Das 9438690103
12 DR MD MUNTAKHAB 8603896453
13 Dr Pratheek Chandrapolla 9848168587
14 Dr Priya Paul P 8281823534
15 Dr Rafath Jahan 9390315675
16 Dr Rik Swarnakar 7003751627
17 Dr Roshiny Rajendran 9048573003
18 Dr Sabina Lois 9790288777
19 Dr Sarath Krishnan M P 9400789781
20 Dr Shruthi Rai P 8073331520
21 Dr Sruthi Sugathan 9746834433
22 Dr Veera Anusha V 9751507332
23 Dr. Sarika Baku 9106639600
24 Dr. Sarvatnida Shaikh 9898350352
25 Dr.Saswati Satpathy 9583152720
26 Dr Jagannadha Phaneendra DS 9866582637
27 Dr T Sony Susan 9701443782
28 Dr JISHMA M 9495202107

About the Workshop: Though born healthy, most babies may have an underlying disorder that may be life-threatening or may lead to permanent mental retardation, if not detected immediately after birth. Screening of newborns will help in the early diagnosis and treatment of these disorders and thus prevent adverse outcomes in the baby. Newborn screening programs are mandatory in all developed countries, but unfortunately not so in our country. Prenatal screening with biochemical markers to detect the pregnant women at risk of carrying a fetus with trisomy as early as in the first trimester will be discussed in detail. The positive screen tests will be further confirmed by amniocentesis or chorionic villous sampling and carrying out karyotyping or fluorescent-in-situ hybridization to detect structural or numerical chromosomal disorders. This workshop aims to train the delegates on various tests that can be performed on newborns or pregnant women to identify the underlying disease condition, their interpretation, quality improvement, communicating with families and the public, and short- and long-term follow-up.

Topic Venue Company-sponsored
Prenatal and Newborn genetics
Day 1 Newborn screening Vani Vilas Women and Children Hospital Perkin Elmer,
Day 2 Prenatal screening (Double, triple, Quadraple and penta markers)
Day 3 Karyotyping & FISH Cytogenetic Division, Ramaiah Medical College, Bengaluru Metasystems

1 Dr. Dhinesh G 9629948536
2 Dr Emi Goldy E 8111098107
3 Dr. R. Prem Richardson 8667807185
4 Dr Ashok Samraj M 9952745941
5 Dr Saranya Raj 8547132599
6 Dr Tharankini Ashokan 8015807004
7 Dr. S Kiruba 9894099607
8 Dr Geerthana 9500550136
9 Dr.TAMILARASI. J 8825663911
10 Dr Athullia Lawrence 7904389031
11 Dr Doney Manuel John 9048026725
12 Dr J. JOHN SAMUEL WEIZMAN 6379537216
13 Dr. Sangeeta Kakkar 9911300538
14 Dr Sushmi Rekha Panda 7978364272
15 Dr ADLA prashanthi 9985943988
16 Dr Sabbavarapu Harika 9100506507
17 Dr Kolli Srujan Kanth 8179416019
18 Dr Soumya Babbili 8790868312
19 Dr Praveen Kumar G 9885172703
20 Dr Suresh Babu 9791430805
21 Dr Ravi Kishore 9980663298
22 Dr Pushpanjali H 8197381581
23 Dr Rashni B J 9980965436
24 Dr Pranshu Dhingra 9462778389
25 Dr Sana Majeed 6238182411
26 Dr Tanya Mishra 7355114282
27 Dr Keerthy Rethinam M 9597790738
28 Dr Sibi Mandela 9035993848
29 Dr Isha Raaj 8248750751

About the Workshop: In the last decade or so Next Generation Sequencing (NGS) technologies have progressively seen a rising shift towards multiple fields including oncology, genetic disorders, and infectious diseases. It has been widely used for genetic testing and disease diagnosis, as well as in predictive, preventive, personalized, and participatory medicine approach. The associated costs have also dropped over the years. This also makes it important to create awareness on the fundamentals, theory and practical utility of the technology.

Topic Venue Company-sponsored
Molecular Diagnostics
Day 1 PCR & qPCR Microbiological Laboratories Thermofischer Scientific
Day 2 Sample preparation & Library preparation
Day 3 Sanger sequencing & Next Generation sequencing

1 Dr Gayathri 9100157507
2 Dr Yar Mohammad Ansari 9893919541
3 Dr Nithya Shankar 9946579238
4 Dr Seeram Sameera 9652245962
5 Dr Joyce Susan Jose 8111934981
6 Dr Ravindra Saxena 9424668293
7 Dr Monisha J 7397262605
8 Dr Saikiran D 8712385108
9 Dr Regi P Solomon 8807428613
10 Dr Rahul Dubey 9407826788
11 Dr K Chandrakala 9487879692
12 Dr Diksha Chandrakar 8319119369
13 Dr Suchanada Sahu 9438884031
14 Dr Manjunath Goud 9.71554E+11

About the Workshop: Liquid chromatography-tandem mass spectrometry (LCMS/MS) is now widely used in clinical laboratories. It enables direct identification of molecules based on the mass- to-charge ratio as well as fragmentation patterns. It offers accurate and reliable results for challenging assays. Advantage of this technology is that we would be able to capture a multitude of information within a single assay. It also allows the detection of compounds at low and high concentrations without any additional sample preparation. This would facilitate analysis of large number of analytes in a single injection, reducing preanalytical steps required and enabling faster time to result. Despite the broad range of clinical applications, its adoption in various clinical laboratories has faced various obstacles probably due to lack of basic understanding of the clinical applications or skilled personnel. Hence this workshop aims to empower the biochemists across the country towards the working principles and the application of this technology in their clinical laboratories.

The AMBI in association with Sciex Center of Excellence would be organizing the workshop at the state-of-the-art facility in the industrial suburbs of Bengaluru which would allow participants to use and apply the technologies on advanced newborn screening, Bioavailability/Bioequivalence (BA/BE) Studies, Metabolomics studies

Topic Venue Company-sponsored
Prenatal and Newborn genetics
Day 1 Basics of Mass Spectrometry Introduction to Triple Quad Demonstration of scan functions, data analysis (SIM, Product ion spectra, neutral loss scan, MRM) Quantification method development SCIEX (A DHR Holding India Pvt. Ltd.) Plot No. 37/3, Hitech/ Defence Aerospace IT Sector, Mahadevakodigehalli Village, Hobli Jala Taluk, Bangalore North, Bangalore- 562149 SCIEX
Day 2 Triple Quad based quantification method QTRAP scan functions and demonstration of a workflow
Day 3 HRMS Scan functions and workflows LC-MS/MS application Overview

1 Dr Mounika Mummadi 9515721802
2 Dr. Andystar Syiemlieh 7005109197
3 Dr Kishor Pawar 8879990394
4 Dr Nayana SM 9652654866
5 Dr Nithin Santhosh 7736493968
6 Dr Kolla Someswara Rao 8328083482
7 Dr Sudheshna Rout 7735114925
8 Dr Srividhya Nandagopal 8940989890
9 Dr Yogabhrintha 9095779797
10 Dr.K.Divya Malar 9566182359
11 Dr Saif Ahmed Khan 8106423838
12 Dr Prakash S 7299062284
13 Dr Kalaivani Sekaran 9489230920
14 Dr Jemima Ajitha P 7401008383
15 Dr Jyoti Sharma 9760852219
16 Dr Surajj SA 9842993306
17 Dr Roshnara P P 9544236516
18 Dr Janani Ramesh 9962072035
19 Dr Akshatha.N.S. 9902244272
20 Dr Keerthy SA 8825604005
21 Dr Shubangi Rathod 9429882792
22 Dr Uma Maheshwari 8870497001

About the workshop: Flow cytometry technique is used to identify and quantify cell population in a heterogenous sample like blood, bone marrow or lymph. These cell subsets are measured by labelling population-specific proteins with a fluorescent tag on the cell surface. In clinical labs, immunophenotyping is useful in diagnosing haematological malignancies such as lymphomas and leukaemia. Specialised flow cytometers also can physically isolate the cells of interest in separate collection tubes that can later be used for research purposes. Two distinct types of cell death, apoptosis, and necrosis can be distinguished by flow cytometry on the basis of differences in morphological, biochemical and molecular changes occurring in the dying cells. This workshop aims to train the delegates on the basics of flow cytometry and utilising this technique for their research as well as routine clinical diagnostics.

Topic Venue Company-sponsored
Flow Cytometry
Day 1 Basics of flow cytometry Quality control and assurance Flow cytometry sample preparation Department of Clinical Pathology, St. Johns Medical College, Bangalore BD
Day 2 Multicolour panel designing Compensation Cell cycle, cell signaling & apoptosis
Day 3 Clinical flow cytometry Data analysis & presentation

1 Dr.Keerthi 9486030744
2 Dr Ephraim Joe 8075247916
3 Dr Ananyaa Dixit 7387584393
4 Dr Saiesh Mardolkar 7841020523
5 Dr Swarna Latha 8978576485
6 Dr Padmasree Paddu 8790290352
7 Dr Tanu Varshney 7078104822
8 Dr.Thashreefa Olakara 9562650830
9 Dr Shubha N Prakash 9741101334
10 Dr Yakalaksmi Velugu 9703030749
11 Dr Sudhahar T 9843373639

Pre-conference workshop on “Advanced techniques in Molecular Biology”
18th to 20th July, 2022
Conducted by
Department of Human Genetics
National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru

Day 1, Monday, 18.07.2022
Time Laboratory of Molecular Therapeutics, Department of Human Genetics, NIMHANS
09.00-11.00 a.m. Opening remarks / Lecture 1 (Lecture Hall 1 – Administrative building, NIMHANS)
11.00-11.30 a.m. RNA isolation from the mammalian cells
11.30-11.45 a.m. Tea/Coffee break
11.45-1.00 p.m. RNA quantification and agarose gel electrophoresis
1.00-2.00 p.m. Lunch break
2.00-3.30 p.m. cDNA conversion / Protein isolation and estimation
3.30-3.45 p.m. Tea/Coffee break
3.45-5.00 p.m. RT-PCR amplification
Note: Participants will be split into Group A and Group B for hands-on training.
Person In Charge: Mrs. M. Abinayaselvi (Group A) and Group B: Mr. M. Narenkumar (Group B)

Day 2, Tuesday, 19.07.2022
Time Laboratory of Molecular Therapeutics, Department of Human Genetics
09.00-10.00 a.m. Lecture 2 (Lecture Hall 1 – Administrative building, NIMHANS)
10.00-11.30 a.m. Sample Preparation and SDS-poly acrylamide gel electrophoresis (SDS-PAGE)
11.30-11.45 a.m. Tea/Coffee break
11.45-1.00 p.m. DNA isolation and quantification
1.00-2.00 p.m. Lunch break
2.00-3.30 p.m. Protein transfer to membrane, blocking / RT-PCR analysis
3.30-3.45 p.m. Tea/Coffee break
3.45-5.00 p.m. Antibody incubation for western blot / PCR amplification from DNA template

Day 3, Wednesday, 20.07.2022
Time Laboratory of Molecular Therapeutics, Department of Human Genetics
09.00-10.00 a.m. Lecture 3 (Lecture Hall 1 – Administrative building, NIMHANS)
10.00-11.30 a.m. Agarose gel Preparation and PCR Analysis
11.30-11.45 am Tea/Coffee break
11.45-12.30 p.m. Western blot analysis
1.00-2.00 p.m. Lunch break
2.00-3.00 p.m. Interaction with event organizer
3:30-4:30 p.m. Concluding remarks

1 Dr Rama prabha 9442921932
2 Dr Judith Sheba Samuel
3 Dr Neethu Joseph 8281154879
4 Dr Devanatha Desikan 8903522594
5 Dr Kowshik Deivasigamani 9994673876
6 Dr Jeyabharathi Dharamlingam 9944955001
7 Dr Kavitha J 9160174724
8 Dr Rasha Nazir Srajudeen 9947347161
9 Dr Sri krishna Paul 9436362580
10 Dr Yamini Mogarala 7892337911
11 Dr P Radhalakshmi 9489230920
12 Dr Meena T 9847743073
13 Dr Gresshma Ajit 8891030090
14 Dr Yogabhrintha 9095779797
15 Dr Hajra Luqman 8019244930

Centre for Human Genetics
AMBICON Preconference Workshop
Clinical Cytogenetics and Molecular Genetics
18th July 2022 to 20th July 2022

Clinical Cytogenetics

About the workshop : Chromosomal abnormalities are genetic aberrations involving a change in chromosome number or structure. Cytogenetics involves studying patient's chromosomes using prenatal, postnatal as well as cancer samples. The techniques employed in studying patient samples include conventional or classical chromosome studies such as routine karyotyping, high resolution banding, NOR staining, C-banding, etc. as well as high end molecular cytogenetic techniques like FISH, PRINS, SKY, mFISH & mBAND FISH. Analysis of the test results is done using appropriate software in the karyotyping and FISH workstation (Metasystem). This workshop aims at sharing knowledge and practical experience in basic cell culture and cytogenetic techniques.

Programme Schedule
Monday, 18th July 2022
Time Contents
10.00 - 10.05 am Welcome note
10.05 - 10.10 am Presentation of the program
10.10 - 10.30 am Lecture 1: ́"Clinical cytogenetics"
10.30 - 11.00 am Lecture 2: ́"Basic Cell culture and Cell transformation Techniques"
11.00 - 11.15 am Lunch break
11.15 - 1.00 pm Lab Session 1:
TC Lab Requirements
Culture setup
Maintenance of culture, subculture and cryopreservation
Harvesting of cultures for cytogenetic testing
Slide preparation
1.00 - 2.00 pm Lunch
2.00 - 2.30 pm Lecture 3: ́"Conventional Cytogenetics Techniques"
2.30 - 3.30 pm Lab Session 2:
G-Banding demonstration
Chromosomal landmarks
Abnormal Karyotypes and ISCN nomenclature
Normal Variations 2 C and NOR banding
Chromosome breakage studies
Karyotype Workstation
3.30 - 3.45 pm Coffee break
3.45 - 4.15 pm Lecture 4: ́"Molecular Cytogenetics"
4.15 - 5.00 pm Lab Session 3:
Direct processing of samples
FISH hybridization
Tuesday, 19th July 2022
Time Contents
10.00 - 11.00 am Lab Session 1:
Post hybridization washing
11.00 - 11.15 am Coffee break
11.15 - 12.30 pm FISH Analysis using workstation
Abnormal FISH cases
12.30 - 1.00 pm Lecture: ́"Reporting and Quality assurance"
11.00-2.00pm Lunch
11.15 - 1.00 pm Lab Session 1:
TC Lab Requirements
Culture setup
Maintenance of culture, subculture and cryopreservation
Harvesting of cultures for cytogenetic testing
Slide preparation
1.00 - 2.00 pm Lunch

Molecular Genetics

About the workshop : Genes are the basic unit of heredity. They carry genetic information in the form of DNA which can be translated into useful proteins to carry out cellular functions. DNA is made up of four bases (abbreviated A, T, C, and G) that are repeated many times throughout a genome and the particular order of this is a critical feature. The human genome, has 3 billion pairs of bases consisting of around 30,000 genes that code for proteins. Genetic disorders are due to alterations in the genome of an organism. A variation or change in the DNA sequence of a gene leads to altered protein that can no longer carry out its normal function, and hence results in a genetic disorder.

Molecular genetics is an investigative approach that involves the application of laboratory methods to investigate genetic disorders at the molecular level. The various techniques used to study the alterations in a gene include Polymerase chain reaction (PCR), DNA Sanger Sequencing and many more. PCR helps generate millions of copies of a specific segment of DNA and Sanger Sequencing "reads" each base of the DNA to find changes that may cause or affect risk for a disease. This workshop aims at covering basic molecular genetics techniques like DNA isolation from peripheral blood, PCR and Sanger Sequencing.

Programme Schedule
Tuesday, 19th July 2022
Time Contents
2.00 - 2.20pm DNA Diagnostics : An overview
2.20 - 3.30pm Lab Session : DNA Isolation from peripheral blood
3.30 - 3.45pm Coffee break
3.45 - 5.00 pm Lab Session : DNA Quantification by Gel Electrophoresis and Nanodrop
Wednesday, 20th July 2022
Time Contents
10.00 - 10.30am Lecture: PCR and Primer Design
10.30 - 11.30am Lab Session 1:
PCR Set up
11.30 - 11.45am Coffee break
11.45 - 12.10pm Quantitative PCR and ARMS PCR
12.10 - 1.00pm Sequence analysis and Report format
1.00-2.00pm Lunch
2.00 - 2.20pm Lecture : Sanger Sequencing
2.20 - 3.30pm Lab Session : DNA Sanger sequencing
3.30 - 3.45pm Coffee break
3.45 - 5.00 pm Q & A Session

1 Dr Shaik Alima Parveen 8374289908
2 Dr Krishna Manasa 9704125091
3 Dr Rohit S 9600642164
4 Dr Nakul Gupta 9729186991
5 Dr Y H Hadassah 7701955607
6 Dr Amarpreet Kaur 6283760762
7 Dr Swati 6201814041
8 Dr Sahera Banu (Dr Munvar Fathima) 7893579106
9 Dr. Madhuti Chakraborty 9433009050
10 Dr Gayathri Devi 9994083717
11 Dr.Prathyusha Prathivadi Bhayankara 8008043720
12 Dr Perkari Anusha Kumari 9866086425
13 Dr Sumathi M E 9749371006
14 Dr Varsha Verma 7771873355
15 Dr Haritha Vishwanathan 8590672170
16 Dr Ankepally Pravallika 8790661343
17 Dr Kishor Srinivas 9744463404
18 Dr Priya Satheesh 9447710206
19 Dr Aparna Laishram 9366146442
20 Dr Anaswara D U 8129261983
21 Dr Diksha Chandrakar 8319119369
22 Dr Shailata Prisi 7903593242
23 Dr Sarvabhavi Kumavat 8108675525
24 Dr Mitali Roy 6001158134
25 Dr. D. Abarna Gnanambigai 9994342506

S.No. Details
1. Glioblastoma cell lines and primary brain tumor culture
2. Proliferation and cytotoxicity assay
3. Migration assay for cancer cells
4. SDS PAGE and Agarose gel electrophoresis
5. Gelatin Zymography for MMPs
6. Tandem Mass spectrometry and its applications in Inborn error of metabolism

Organized by:
Dr. Nandakumar DN
Professor and Head
Department of Neurochemistry

1 Dr Nimmy Maria James 8289853722
2 Dr Vajja Swathi 8500667235
3 Dr Yamini Mogarala 7892337911
4 Dr Piyush Pathak 8095441135
5 Dr Rashmi Nain 8699840157
6 Dr Rupali Bains 8837871635
7 Dr Sankeerth Palakonda 9542460735
8 Dr Piyush Kumar Singh 9987297668
9 Dr Azarudeen 9597736656
10 Dr Nihaal Maripini 9663110250
11 Dr Nandan Valavaikar 8691905759
12 Dr Utsav Haldar 8910037419
13 Dr Rohini T Prasad 7736388804
14 Dr Jyothi D N 9449779934