About the Workshop: Though born healthy, most babies may have an underlying disorder that may be life-threatening or may lead to permanent mental retardation, if not detected immediately after birth. Screening of newborns will help in the early diagnosis and treatment of these disorders and thus prevent adverse outcomes in the baby. Newborn screening programs are mandatory in all developed countries, but unfortunately not so in our country. Prenatal screening with biochemical markers to detect the pregnant women at risk of carrying a fetus with trisomy as early as in the first trimester will be discussed in detail. The positive screen tests will be further confirmed by amniocentesis or chorionic villous sampling and carrying out karyotyping or fluorescent-in-situ hybridization to detect structural or numerical chromosomal disorders. This workshop aims to train the delegates on various tests that can be performed on newborns or pregnant women to identify the underlying disease condition, their interpretation, quality improvement, communicating with families and the public, and short- and long-term follow-up.

	Topic	Venue	Company-sponsored
Prenatal and Newborn genetics
Day 1	Newborn screening	Vani Vilas Women and Children Hospital	Perkin Elmer,
Day 2	Prenatal screening (Double, triple, Quadraple and penta markers)
Day 3	Karyotyping & FISH	Cytogenetic Division, Ramaiah Medical College, Bengaluru	Metasystems

S.NO	NAMES OF THE PARTICIPANTS	EMAIL ID	Contact No
1	Dr. Dhinesh G	haridhinesh1207@gmail.com	9629948536
2	Dr Emi Goldy E	emigoldy@gmail.com	8111098107
3	Dr. R. Prem Richardson	premrichardson@gmail.com	8667807185
4	Dr Ashok Samraj M	samanand8@gmail.com	9952745941
5	Dr Saranya Raj	dr.saranyaraj.is@gmail.com	8547132599
6	Dr Tharankini Ashokan	tharankini.a@gmail.com	8015807004
7	Dr. S Kiruba	dr.kiruba84@gmail.com	9894099607
8	Dr Geerthana	b.geerthana@yahoo.in	9500550136
9	Dr.TAMILARASI. J	drtamilarasii@gmail.com	8825663911
10	Dr Athullia Lawrence	athullia.lawrence@gmail.com	7904389031
11	Dr Doney Manuel John	doneymanuel79@gmail.com	9048026725
12	Dr J. JOHN SAMUEL WEIZMAN	johnsamuel.weizman07@gmail.com	6379537216
13	Dr. Sangeeta Kakkar	sangkhu28@gmail.com	9911300538
14	Dr Sushmi Rekha Panda	sushmirekhapanda@gmail.com	7978364272
15	Dr ADLA prashanthi	srikanthogh@gmail.com	9985943988
16	Dr Sabbavarapu Harika	harikasabbavarapu@gmail.com	9100506507
17	Dr Kolli Srujan Kanth	srujankanth7@gmail.com	8179416019
18	Dr Soumya Babbili	babbilisoumya@gmail.com	8790868312
19	Dr Praveen Kumar G	praveen.gadmoore@gmail.com	9885172703
20	Dr Suresh Babu	drsuresh703@gmail.com	9791430805
21	Dr Ravi Kishore	writekishore@yahoo.co.in	9980663298
22	Dr Pushpanjali H	mesmer3145@gmail.com	8197381581
23	Dr Rashni B J	dr.rashni.bj@gmail.com	9980965436
24	Dr Pranshu Dhingra	pranshudhingra09@gmail.com	9462778389
25	Dr Sana Majeed	sanamajeed3011@gmail.com	6238182411
26	Dr Tanya Mishra	mishra.tanya5592@gmail.com	7355114282
27	Dr Keerthy Rethinam M	m0420003@sriramachandra.edu.in	9597790738
28	Dr Sibi Mandela	drtsibimandela@aiimsmangalagiri.edu.in	9035993848
29	Dr Isha Raaj	drisharaaj@gmail.com	8248750751

About the Workshop: In the last decade or so Next Generation Sequencing (NGS) technologies have progressively seen a rising shift towards multiple fields including oncology, genetic disorders, and infectious diseases. It has been widely used for genetic testing and disease diagnosis, as well as in predictive, preventive, personalized, and participatory medicine approach. The associated costs have also dropped over the years. This also makes it important to create awareness on the fundamentals, theory and practical utility of the technology.

	Topic	Venue	Company-sponsored
Molecular Diagnostics
Day 1	PCR & qPCR	Microbiological Laboratories	Thermofischer Scientific
Day 2	Sample preparation & Library preparation
Day 3	Sanger sequencing & Next Generation sequencing

S.NO	NAMES OF THE PARTICIPANTS	EMAIL ID	Contact No
1	Dr Gayathri	sudhagayatri88@gmail.com	9100157507
2	Dr Yar Mohammad Ansari	dryaransari724@gmail.com	9893919541
3	Dr Nithya Shankar	nithyajs@gmail.com	9946579238
4	Dr Seeram Sameera	sameera78062@gmail.com	9652245962
5	Dr Joyce Susan Jose	joycesusanjose@gmail.com	8111934981
6	Dr Ravindra Saxena	ravisaxena72@gmail.com	9424668293
7	Dr Monisha J	monipriya2231997@gmail.com	7397262605
8	Dr Saikiran D	dungi.saikiran@gmail.com	8712385108
9	Dr Regi P Solomon	regiusccxr@gmail.com	8807428613
10	Dr Rahul Dubey	rahuldubeylover66@gmail.com	9407826788
11	Dr K Chandrakala	chameka14@gmail.com	9487879692
12	Dr Diksha Chandrakar	drdikshachandrakar@gmail.com	8319119369
13	Dr Suchanada Sahu	biochem_suchanda@aiimsbhubaneswar.edu.in	9438884031
14	Dr Manjunath Goud	drmanjunathag@gmail.com	9.71554E+11

About the Workshop: Liquid chromatography-tandem mass spectrometry (LCMS/MS) is now widely used in clinical laboratories. It enables direct identification of molecules based on the mass- to-charge ratio as well as fragmentation patterns. It offers accurate and reliable results for challenging assays. Advantage of this technology is that we would be able to capture a multitude of information within a single assay. It also allows the detection of compounds at low and high concentrations without any additional sample preparation. This would facilitate analysis of large number of analytes in a single injection, reducing preanalytical steps required and enabling faster time to result. Despite the broad range of clinical applications, its adoption in various clinical laboratories has faced various obstacles probably due to lack of basic understanding of the clinical applications or skilled personnel. Hence this workshop aims to empower the biochemists across the country towards the working principles and the application of this technology in their clinical laboratories.

The AMBI in association with Sciex Center of Excellence would be organizing the workshop at the state-of-the-art facility in the industrial suburbs of Bengaluru which would allow participants to use and apply the technologies on advanced newborn screening, Bioavailability/Bioequivalence (BA/BE) Studies, Metabolomics studies

	Topic	Venue	Company-sponsored
Prenatal and Newborn genetics
Day 1	Basics of Mass Spectrometry Introduction to Triple Quad Demonstration of scan functions, data analysis (SIM, Product ion spectra, neutral loss scan, MRM) Quantification method development	SCIEX (A DHR Holding India Pvt. Ltd.) Plot No. 37/3, Hitech/ Defence Aerospace IT Sector, Mahadevakodigehalli Village, Hobli Jala Taluk, Bangalore North, Bangalore- 562149	SCIEX
Day 2	Triple Quad based quantification method QTRAP scan functions and demonstration of a workflow
Day 3	HRMS Scan functions and workflows LC-MS/MS application Overview

S.NO	NAMES OF THE PARTICIPANTS	EMAIL ID	Contact No
1	Dr Mounika Mummadi	drmounika227@gmail.com	9515721802
2	Dr. Andystar Syiemlieh	astrowhite130@gmail.com	7005109197
3	Dr Kishor Pawar	drkishorpawar@gmail.com	8879990394
4	Dr Nayana SM	nayanadevang@gmail.com	9652654866
5	Dr Nithin Santhosh	nithin.santhosh.23@gmail.com	7736493968
6	Dr Kolla Someswara Rao	somesh.kolla@gmail.com	8328083482
7	Dr Sudheshna Rout	sudeshnarout.mmch@gmail.com	7735114925
8	Dr Srividhya Nandagopal	Srividhyanandagopal@gmail.com	8940989890
9	Dr Yogabhrintha	yogabhrintha@gmail.com	9095779797
10	Dr.K.Divya Malar	drdivyamalar92@gmail.com	9566182359
11	Dr Saif Ahmed Khan	saifdkhan2000@gmail.com	8106423838
12	Dr Prakash S	drpkash@gmail.com	7299062284
13	Dr Kalaivani Sekaran	kalaisekaran90@gmail.com	9489230920
14	Dr Jemima Ajitha P	drjemimaajitha@gmail.com	7401008383
15	Dr Jyoti Sharma	jazzyjoe.sharma4@gmail.com	9760852219
16	Dr Surajj SA	surajj.surajj@gmail.com	9842993306
17	Dr Roshnara P P	drroshnarapp@gmail.com	9544236516
18	Dr Janani Ramesh	janani13r@gmail.com	9962072035
19	Dr Akshatha.N.S.	akshu.n.s768@gmail.com	9902244272
20	Dr Keerthy SA	drkeerthy04@gmail.com	8825604005
21	Dr Shubangi Rathod	dr.shubhangirathod@gmail.com	9429882792
22	Dr Uma Maheshwari	dr.umamaheswarib@gmail.com	8870497001

About the workshop: Flow cytometry technique is used to identify and quantify cell population in a heterogenous sample like blood, bone marrow or lymph. These cell subsets are measured by labelling population-specific proteins with a fluorescent tag on the cell surface. In clinical labs, immunophenotyping is useful in diagnosing haematological malignancies such as lymphomas and leukaemia. Specialised flow cytometers also can physically isolate the cells of interest in separate collection tubes that can later be used for research purposes. Two distinct types of cell death, apoptosis, and necrosis can be distinguished by flow cytometry on the basis of differences in morphological, biochemical and molecular changes occurring in the dying cells. This workshop aims to train the delegates on the basics of flow cytometry and utilising this technique for their research as well as routine clinical diagnostics.

	Topic	Venue	Company-sponsored
Flow Cytometry
Day 1	Basics of flow cytometry Quality control and assurance Flow cytometry sample preparation	Department of Clinical Pathology, St. Johns Medical College, Bangalore	BD
Day 2	Multicolour panel designing Compensation Cell cycle, cell signaling & apoptosis
Day 3	Clinical flow cytometry Data analysis & presentation

S.NO	NAMES OF THE PARTICIPANTS	EMAIL ID	Contact No
1	Dr.Keerthi	keerthiponarasu@gmail.com	9486030744
2	Dr Ephraim Joe	ephraimjoe96@gmail.com	8075247916
3	Dr Ananyaa Dixit	ad.30393@gmail.com	7387584393
4	Dr Saiesh Mardolkar	saiesh44@gmail.com	7841020523
5	Dr Swarna Latha	dr.swarna2002@gmail.com	8978576485
6	Dr Padmasree Paddu	padmasreepaddu28@gmail.com	8790290352
7	Dr Tanu Varshney	omtanuvarshney@gmail.com	7078104822
8	Dr.Thashreefa Olakara	thashreefa@gmail.com	9562650830
9	Dr Shubha N Prakash	shubha.np@stjohns.in	9741101334
10	Dr Yakalaksmi Velugu	drsainandan26@gmail.com	9703030749
11	Dr Sudhahar T	sudhahar1216@gmail.com	9843373639

Pre-conference workshop on “Advanced techniques in Molecular Biology”
18^th to 20^th July, 2022
Conducted by
Department of Human Genetics
National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru

Day 1, Monday, 18.07.2022
Time	Laboratory of Molecular Therapeutics, Department of Human Genetics, NIMHANS
09.00-11.00 a.m.	Opening remarks / Lecture 1 (Lecture Hall 1 – Administrative building, NIMHANS)
11.00-11.30 a.m.	RNA isolation from the mammalian cells
11.30-11.45 a.m.	Tea/Coffee break
11.45-1.00 p.m.	RNA quantification and agarose gel electrophoresis
1.00-2.00 p.m.	Lunch break
2.00-3.30 p.m.	cDNA conversion / Protein isolation and estimation
3.30-3.45 p.m.	Tea/Coffee break
3.45-5.00 p.m.	RT-PCR amplification
Note: Participants will be split into Group A and Group B for hands-on training. Person In Charge: Mrs. M. Abinayaselvi (Group A) and Group B: Mr. M. Narenkumar (Group B)

Day 2, Tuesday, 19.07.2022
Time	Laboratory of Molecular Therapeutics, Department of Human Genetics
09.00-10.00 a.m.	Lecture 2 (Lecture Hall 1 – Administrative building, NIMHANS)
10.00-11.30 a.m.	Sample Preparation and SDS-poly acrylamide gel electrophoresis (SDS-PAGE)
11.30-11.45 a.m.	Tea/Coffee break
11.45-1.00 p.m.	DNA isolation and quantification
1.00-2.00 p.m.	Lunch break
2.00-3.30 p.m.	Protein transfer to membrane, blocking / RT-PCR analysis
3.30-3.45 p.m.	Tea/Coffee break
3.45-5.00 p.m.	Antibody incubation for western blot / PCR amplification from DNA template

Day 3, Wednesday, 20.07.2022
Time	Laboratory of Molecular Therapeutics, Department of Human Genetics
09.00-10.00 a.m.	Lecture 3 (Lecture Hall 1 – Administrative building, NIMHANS)
10.00-11.30 a.m.	Agarose gel Preparation and PCR Analysis
11.30-11.45 am	Tea/Coffee break
11.45-12.30 p.m.	Western blot analysis
1.00-2.00 p.m.	Lunch break
2.00-3.00 p.m.	Interaction with event organizer
3:30-4:30 p.m.	Concluding remarks

S.NO	NAMES OF THE PARTICIPANTS	EMAIL ID	Contact No
1	Dr Rama prabha	sakthiram9923@gmail.com	9442921932
2	Dr Judith Sheba Samuel	judith27samuel@gmail.com
3	Dr Neethu Joseph	nijosne@gmail.com	8281154879
4	Dr Devanatha Desikan	devanathadesikan@outlook.com	8903522594
5	Dr Kowshik Deivasigamani	kowshik.deivasigamani@gmail.com	9994673876
6	Dr Jeyabharathi Dharamlingam	vmdbharathi@gmail.com	9944955001
7	Dr Kavitha J	kavitha.jky@gmail.com	9160174724
8	Dr Rasha Nazir Srajudeen	rasha.nazir@gmail.com	9947347161
9	Dr Sri krishna Paul	skpaul.agt@gmail.com	9436362580
10	Dr Yamini Mogarala	msy.mini@gmail.com	7892337911
11	Dr P Radhalakshmi	rlpnayagam@gmail.com	9489230920
12	Dr Meena T	meenat05@gmail.com	9847743073
13	Dr Gresshma Ajit	greeshmajith2010@gmail.com	8891030090
14	Dr Yogabhrintha	yogabhrintha@gmail.com	9095779797
15	Dr Hajra Luqman	dr.hajraluqman3@gmail.com	8019244930

Centre for Human Genetics
Bengaluru
AMBICON Preconference Workshop
Clinical Cytogenetics and Molecular Genetics
18^th July 2022 to 20^th July 2022

Clinical Cytogenetics

About the workshop : Chromosomal abnormalities are genetic aberrations involving a change in chromosome number or structure. Cytogenetics involves studying patient's chromosomes using prenatal, postnatal as well as cancer samples. The techniques employed in studying patient samples include conventional or classical chromosome studies such as routine karyotyping, high resolution banding, NOR staining, C-banding, etc. as well as high end molecular cytogenetic techniques like FISH, PRINS, SKY, mFISH & mBAND FISH. Analysis of the test results is done using appropriate software in the karyotyping and FISH workstation (Metasystem). This workshop aims at sharing knowledge and practical experience in basic cell culture and cytogenetic techniques.

Programme Schedule

Monday, 18th July 2022
Time	Contents
10.00 - 10.05 am	Welcome note
10.05 - 10.10 am	Presentation of the program
10.10 - 10.30 am	Lecture 1: ́"Clinical cytogenetics"
10.30 - 11.00 am	Lecture 2: ́"Basic Cell culture and Cell transformation Techniques"
11.00 - 11.15 am	Lunch break
11.15 - 1.00 pm	Lab Session 1: TC Lab Requirements Culture setup Maintenance of culture, subculture and cryopreservation Harvesting of cultures for cytogenetic testing Slide preparation
1.00 - 2.00 pm	Lunch
2.00 - 2.30 pm	Lecture 3: ́"Conventional Cytogenetics Techniques"
2.30 - 3.30 pm	Lab Session 2: G-Banding demonstration Chromosomal landmarks Abnormal Karyotypes and ISCN nomenclature Normal Variations 2 C and NOR banding Chromosome breakage studies Karyotype Workstation
3.30 - 3.45 pm	Coffee break
3.45 - 4.15 pm	Lecture 4: ́"Molecular Cytogenetics"
4.15 - 5.00 pm	Lab Session 3: Direct processing of samples FISH hybridization

Tuesday, 19th July 2022
Time	Contents
10.00 - 11.00 am	Lab Session 1: Post hybridization washing
11.00 - 11.15 am	Coffee break
11.15 - 12.30 pm	FISH Analysis using workstation Abnormal FISH cases
12.30 - 1.00 pm	Lecture: ́"Reporting and Quality assurance"
11.00-2.00pm	Lunch
11.15 - 1.00 pm	Lab Session 1: TC Lab Requirements Culture setup Maintenance of culture, subculture and cryopreservation Harvesting of cultures for cytogenetic testing Slide preparation
1.00 - 2.00 pm	Lunch

Molecular Genetics

About the workshop : Genes are the basic unit of heredity. They carry genetic information in the form of DNA which can be translated into useful proteins to carry out cellular functions. DNA is made up of four bases (abbreviated A, T, C, and G) that are repeated many times throughout a genome and the particular order of this is a critical feature. The human genome, has 3 billion pairs of bases consisting of around 30,000 genes that code for proteins. Genetic disorders are due to alterations in the genome of an organism. A variation or change in the DNA sequence of a gene leads to altered protein that can no longer carry out its normal function, and hence results in a genetic disorder.

Molecular genetics is an investigative approach that involves the application of laboratory methods to investigate genetic disorders at the molecular level. The various techniques used to study the alterations in a gene include Polymerase chain reaction (PCR), DNA Sanger Sequencing and many more. PCR helps generate millions of copies of a specific segment of DNA and Sanger Sequencing "reads" each base of the DNA to find changes that may cause or affect risk for a disease. This workshop aims at covering basic molecular genetics techniques like DNA isolation from peripheral blood, PCR and Sanger Sequencing.

Programme Schedule

Tuesday, 19th July 2022
Time	Contents
2.00 - 2.20pm	DNA Diagnostics : An overview
2.20 - 3.30pm	Lab Session : DNA Isolation from peripheral blood
3.30 - 3.45pm	Coffee break
3.45 - 5.00 pm	Lab Session : DNA Quantification by Gel Electrophoresis and Nanodrop

Wednesday, 20th July 2022
Time	Contents
10.00 - 10.30am	Lecture: PCR and Primer Design
10.30 - 11.30am	Lab Session 1: PCR Set up
11.30 - 11.45am	Coffee break
11.45 - 12.10pm	Quantitative PCR and ARMS PCR
12.10 - 1.00pm	Sequence analysis and Report format
1.00-2.00pm	Lunch
2.00 - 2.20pm	Lecture : Sanger Sequencing
2.20 - 3.30pm	Lab Session : DNA Sanger sequencing
3.30 - 3.45pm	Coffee break
3.45 - 5.00 pm	Q & A Session

S.NO	NAMES OF THE PARTICIPANTS	EMAIL ID	Contact No
1	Dr Shaik Alima Parveen	shaikparveenmbbs@gmail.com	8374289908
2	Dr Krishna Manasa	krishnamanasar38@gmail.com	9704125091
3	Dr Rohit S	drrohit1994@gmail.com	9600642164
4	Dr Nakul Gupta	nakul09gupta@gmail.com	9729186991
5	Dr Y H Hadassah	pretty.hadassahdalz@gmail.com	7701955607
6	Dr Amarpreet Kaur	preetamar418@gmail.com	6283760762
7	Dr Swati	swatisingh17392@gmail.com	6201814041
8	Dr Sahera Banu (Dr Munvar Fathima)	saherabanu117@gmail.com	7893579106
9	Dr. Madhuti Chakraborty	thisismadhuti@gmail.com	9433009050
10	Dr Gayathri Devi	gayathrishiwalik@gmail.com	9994083717
11	Dr.Prathyusha Prathivadi Bhayankara	drpbprathyusha@gmail.com	8008043720
12	Dr Perkari Anusha Kumari	anusha.perkari@gmail.com	9866086425
13	Dr Sumathi M E	drsuma_bio@yahoo.co.in	9749371006
14	Dr Varsha Verma	vershv@gmail.com	7771873355
15	Dr Haritha Vishwanathan	haritha.v999@gmail.com	8590672170
16	Dr Ankepally Pravallika	epravallikareddy@gmail.com	8790661343
17	Dr Kishor Srinivas	kishorsrini@gmail.com	9744463404
18	Dr Priya Satheesh	priyasatheesh64@gmail.com	9447710206
19	Dr Aparna Laishram	aprlaishram@gmail.com	9366146442
20	Dr Anaswara D U	anaswaradevu@gmail.com	8129261983
21	Dr Diksha Chandrakar	drdikshachandrakar@gmail.com	8319119369
22	Dr Shailata Prisi	drshailataprisi@gmail.com	7903593242
23	Dr Sarvabhavi Kumavat	drsarvabhavi@yahoo.co.in	8108675525
24	Dr Mitali Roy	mitaliroy97@gmail.com	6001158134
25	Dr. D. Abarna Gnanambigai	abarnagnanambigai1989@gmail.com	9994342506

S.No.	Details
1.	Glioblastoma cell lines and primary brain tumor culture
2.	Proliferation and cytotoxicity assay
3.	Migration assay for cancer cells
4.	SDS PAGE and Agarose gel electrophoresis
5.	Gelatin Zymography for MMPs
6.	Tandem Mass spectrometry and its applications in Inborn error of metabolism

Organized by:
Dr. Nandakumar DN
Professor and Head
Department of Neurochemistry
NIMHANS
Bengaluru

S.NO	NAMES OF THE PARTICIPANTS	EMAIL ID	Contact No
1	Dr Nimmy Maria James	nimmyjames143@gmail.com	8289853722
2	Dr Vajja Swathi	dr.vajja@gmail.com	8500667235
3	Dr Yamini Mogarala	msy.mini@gmail.com	7892337911
4	Dr Piyush Pathak	piyush.pathak88@gmail.com	8095441135
5	Dr Rashmi Nain	rashminain1995@gmail.com	8699840157
6	Dr Rupali Bains	rupali.prinston@gmail.com	8837871635
7	Dr Sankeerth Palakonda	sankychary11@gmail.com	9542460735
8	Dr Piyush Kumar Singh	p311ks@gmail.com	9987297668
9	Dr Azarudeen	ks.aslam@gmail.com	9597736656
10	Dr Nihaal Maripini	nmaripini@gmail.com	9663110250
11	Dr Nandan Valavaikar	valavaikarnandan@gmail.com	8691905759
12	Dr Utsav Haldar	utsavh07@gmail.com	8910037419
13	Dr Rohini T Prasad	rohintprasad@gmail.com	7736388804
14	Dr Jyothi D N	jyothidn2009@gmail.com	9449779934